Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 78, Issue -, Pages 40-45Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2015.06.006
Keywords
Graphene; Carbon nanotubes; Gold nanoparticles; Glucose oxidase; Biosensor; Electrochemistry
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Funding
- Ministry of Science and Technology, Taiwan (Republic of China) [NSC 103-2811-M-027-002, 102-2113-M-027-002-MY3]
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Biopolymer pectin stabilized gold nanoparticles were prepared at graphene and multiwalled carbon nanotubes (GR-MWNTs/AuNPs) and employed for the determination of glucose. The formation of GR-MWNTs/AuNPs was confirmed by scanning electron microscopy, X-ray diffraction, UV-vis and FTIR spectroscopy methods. Glucose oxidase (GOx) was successfully immobilized on GR-MWNTs/AuNPs film and direct electron transfer of GOx was investigated. GOx exhibits highly enhanced redox peaks with formal potential of -0.40V (vs. Ag/AgCl). The amount of electroactive GOx and electron transfer rate constant were found to be 10.5 x 10(-10) mol cm(-2) and 336 s(-1), respectively, which were significantly larger than the previous reports. The fabricated amperometric glucose biosensor sensitively detects glucose and showed two linear ranges: (1) 10 mu M - 2 mM with LOD of 4.1 mu M, (2) 2 mM - 5.2 mM with LOD of 0.95 mM. The comparison of the biosensor performance with reported sensors reveals the significant improvement in overall sensor performance. Moreover, the biosensor exhibited appreciable stability, repeatability, reproducibility and practicality. The other advantages of the fabricated biosensor are simple and green fabrication approach, roughed and stable electrode surface, fast in sensing and highly reproducible. (C) 2015 Elsevier Inc. All rights reserved.
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