Journal
MOSCOW UNIVERSITY CHEMISTRY BULLETIN
Volume 72, Issue 6, Pages 269-274Publisher
ALLERTON PRESS INC
DOI: 10.3103/S0027131417060037
Keywords
immunofluorescence analysis; flow cytometry; serous ovarian cancer; ERCC1
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Funding
- Russian Foundation for Basic Research [15-04-06991-a, 16-34-01049-mol-a]
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In the present research, we compared two datasets obtained from the quantitative estimation of ERCC1 protein using two monoclonal antibodies-8F1 and FL29. Quantitative evaluation of immunofluorescence parameters was performed by flow cytometry on ovarian cancer biopsy specimens (n = 53). Agreement between two different assays was estimated by correlation analyses and Bland-Altman plots. It was shown that, despite of large spread of differences etween two measurements obtained by 8F1 and FL297, both clones may be used for estimation of ERCC1 expression level (i.e., the number of positively stained cells) in tumor.
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