Cellular Internalization of Rod-Like Nanoparticles with Various Surface Patterns: Novel Entry Pathway and Controllable Uptake Capacity

The cellular internalization of rod-like nanoparticles (NPs) is investigated in a combined experimental and simulation study. These rod-like nanoparticles with smooth, abacus-like (i.e., beads-on-wires), and helical surface patterns are prepared by the cooperative self-assembly of poly(gamma-benzyl-l-glutamate)-block-poly(ethylene glycol) (PBLG-b-PEG) block copolymers and PBLG homopolymers. All three types of NPs can be internalized via endocytosis. Helical NPs exhibit the best endocytic efficacy, followed by smooth NPs and abacus-like NPs. Coarse-grained molecular dynamics simulations are used to examine the endocytic efficiency of these NPs. The NPs with helical and abacus-like surfaces can be endocytosed via novel standing up (tip entry) and gyroscope-like (precession) pathways, respectively, which are distinct from the pathway of traditional NPs with smooth surfaces. This finding indicates that the cellular internalization capacity and pathways can be regulated by introducing stripe patterns (helical and abacus-like) onto the surface of rod-like NPs. The results of this study may lead to novel applications of biomaterials, such as advanced drug delivery systems.