Journal
SMALL
Volume 13, Issue 41, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201701631
Keywords
heterogeneity; mesoporous silica nanoparticles; protein corona; super-resolution imaging; targeting
Categories
Funding
- AXA research fund
- Spanish Ministry of Economy, Industry and Competitiveness
- Generalitat de Catalunya through the CERCA program
- Spanish Ministry of Economy, Industry and Competitiveness [SAF2016-75241-R]
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The adsorption of serum proteins, leading to the formation of a biomolecular corona, is a key determinant of the biological identity of nanoparticles in vivo. Therefore, gaining knowledge on the formation, composition, and temporal evolution of the corona is of utmost importance for the development of nanoparticle-based therapies. Here, it is shown that the use of super-resolution optical microscopy enables the imaging of the protein corona on mesoporous silica nanoparticles with single protein sensitivity. Particle-by-particle quantification reveals a significant heterogeneity in protein absorption under native conditions. Moreover, the diversity of the corona evolves over time depending on the surface chemistry and degradability of the particles. This paper investigates the consequences of protein adsorption for specific cell targeting by antibody-functionalized nanoparticles providing a detailed understanding of corona-activity relations. The methodology is widely applicable to a variety of nanostructures and complements the existing ensemble approaches for protein corona study.
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