Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 253, Issue -, Pages 660-665Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.06.174
Keywords
Hybridization chain reaction; Personal glucose meter; Invertase; Vascular endothelial growth factor
Funding
- NSFC [21305014, 81202912, 21677033]
- Natural Sciences Foundation of Fujian Province [2014J05013]
- Outstanding Youth Research Training Program of Fujian Agriculture and Forestry University [xjq201409]
- Key Subject of Ecology, Fujian Province [6112C0600]
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In this paper, coupled with hybridization chain reaction and portable personal glucose meter, a highly sensitive aptamer-based immunoassay for vascular endothelial growth factor (VEGF) is fabricated. The antibody immobilized on the microplate captured the target protein, which would be sandwiched by the formation of aptamer/VEGF/antibody complex in the presence of aptamer. And then, an 18-nt elongated strand at the 3' end of aptamer, with the assist of invertase-functionalized auxiliary probe, triggered the hybridization chain reaction to generate invertase-concatamers. Vast invertase catalyzed sucrose to glucose, which is monitored through personal glucose meter. Finally, this portable sensing system can achieve the detection of limit of 1.2 pg/mL with good stability and selectivity. Meanwhile, VEGF in the serum would also be assayed with satisfied result. (C) 2017 Published by Elsevier B.V.
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