Single-step procedure for the modification of graphite electrode by composite layer based on polypyrrole, Prussian blue and glucose oxidase

In this research single-step procedure for the modification of graphite electrode by polypyrrole (PPy), Prussian blue (PB) and glucose oxidase (GOx) based composite layer (PPy/PB/GOx) is reported. The PPy/PB/G0x-modified electrode was applied in the design of glucose biosensor. Optimal potential for the registration of amperometric response towards glucose was at +0.05 V vs Ag/AgCl(KCIsat). Preliminary optimization of initial concentration of pyrrole in initial solution, which was used for the formation of PPy/PB/GOx layer, was performed. The sensitivity of several different PPy/PB/GOx-modified graphite electrodes, which were modified by PPy/PB/GOx layer at different pyrrole concentrations, was evaluated, and it was in a range of 1.0-1.9 mu A cm(-2) mM(-1). (C) 2016 Elsevier B.V. All rights reserved.