Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 246, Issue -, Pages 1049-1055Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.02.150
Keywords
3D Paper-based microfluidics; C-Reactive protein; Pressed paper; Vertical flow assays
Funding
- Mid-Career Researcher Program [NRF-2016R1A2B3015986]
- Bio & Medical Technology Development Program [NRF-2015M3A9B3028685]
- NRF-Global Ph.D. Fellowship Program through National Research Foundation (NRF) of Korea - Ministry of Science, ICT and Future Planning
- National Research Foundation of Korea [2015M3A9B3028685] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Although vertical flow assays (VFAs) have a number of advantages compared to lateral flow assays (LFAs) such as a short analysis time, no line interference, and no Hook effect, VFAs are not preferred as LFAs because of their complicated operation principle. In this study, we demonstrated VFAs with multistep reactions for the detection of C-reactive protein (CRP) based on the programmed reagent loading in a pressed region integrated 3D paper-based microfluidic device. The flow order of all reagents in a 3D paper-based microfluidic device was programmed based on the delayed flow caused by the pressed region as well as the geometry modification of the paper channel. After simultaneous loading of all the reagents required for assays, they are sequentially loaded into the analysis region with a programmed sequence. As a proof of concept, a high-sensitivity CRP (hs-CRP) detection with signal amplification was performed to predict the riskiness of cardiovascular disease within 15 min. The detection limit was improved from 0.01 to 0.005 mu g/mL via a maximum 3.47-fold signal amplification. Additionally, the upper limit of hs-CRP detection increased to 5 mu g/mL without Hook effect. Finally, we successfully detected hs-CRP in a clinically relevant range (0.005-5 mu g/mL), while LFAs cannot cover due to the Hook effect. (C) 2017 Elsevier B.V. All rights reserved.
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