Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 244, Issue -, Pages 585-590Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.01.032
Keywords
Ratiometric fluorescence biosensor; HIV dsDNA; Quantum dots; Mitoxantrone
Funding
- National Natural Science Foundation of China [21275098]
- Natural Science Grand Research Program of Shaanxi Province [2013SZS08-Z01]
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A ratiometric fluorescence biosensor was developed for detection of double strand DNA (dsDNA). The sensor consists of water-soluble fluorescent carbon dots (CDs) and 3-mercapropionic acid-coated cadmium telluride (CdTe) quantum dots exhibiting emissions peaks at 435 and 599 nm, respectively, under single-wavelength excitation (360 nm). CdTe QDs fluorescence was quenched by mitoxantrone via electron transfer and was restored in the presence of dsDNA, while the fluorescence intensity of CDs remained almost constant, providing a ratiometric means of dsDNA detection. The relative fluorescence intensity ration is directly proportional to the concentration of dsDNA between 0 and 50 nM, and the detection limit is 1.0 nM. Meanwhile, common organic compounds including amino acids, nucleotides, bovine serum albumin, single strand DNA and RNA had not significant interference in the detection mode. The novel nanosensor is simple, rapid, and convenient since it does not require modification or separation procedures, and was applied to the detection of HIV dsDNA in synthetic samples and human serum samples with satisfactory results. (C) 2017 Elsevier B.V. All rights reserved.
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