Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 243, Issue -, Pages 1240-1247Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.12.107
Keywords
Primer-containing molecular beacon (PMB); Exponential rolling circle amplification (E-RCA); Tumor suppressor p53 gene
Funding
- National Natural Science Foundation of China (NSFC) [21275002]
- Independent Research Project of State Key Laboratory of Photocatalysis on Energy and Environment [2014CO1]
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The development of robust biosensing platforms for accurate detection of cancer-related gene is of great significance for early diagnosis and treatment of cancer. In this paper, we proposed a new concept, exponential rolling circle amplification (E-RCA), for the circular oligonucleotide-mediated tumor suppressor p53 gene detection. Specifically, PMB (primer-containing molecular beacon), PPT (primer-containing polymerization template) and padlock probe are involved. PMB is capable of recognizing target DNA and serves as signal reporter, while PPT is designed to harbor the half binding site of nicking endonuclease and is used as the, primary polymerization primer. After the target-activated hybridization between PMB and PPT, besides the as-designed PPT can promote the cyclical target strand-displacement polymerization (CTDP) and nicking-mediated strand-displacement polymerization (NSDP), the nicked primer-induced RCA can make subsequent NSDPs and RCAs proceed repeatedly and exponentially, increasing dramatically the optical signal due to the opening of a large amount of PMBs. The p53 gene can be detected down to 1 pM, and point mutation can be easily discriminated. The exciting breakthrough in the genetic diagnosis concept, desirable analytical characteristics and measured data for real sample assay indicate the potential application in biomedical research and early clinical diagnostics. (C) 2016 Elsevier B.V. All rights reserved.
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