Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 240, Issue -, Pages 158-167Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.08.104
Keywords
Electrodeformation; Epithelial-mesenchymal transition; Microchip; Cell viscoelasticity; Cancer diagnosis
Funding
- National Basic Research Program of China [2013CB933702]
- National Natural Science Foundation of China (NSFC) [11472013, 11272015, 11002003]
- Peking University 985 program
Ask authors/readers for more resources
Cancer development and progression associated with epithelial-mesenchymal transition (EMT) not only results in biological and functional abnormalities but also leads to changes in mechanical and structural characteristics of cells. Mechanical characterization has been considered as a promising, label-free, alternative way to cancer diagnosis. In this paper, we fabricate a microchip to quantify mechanical deformability of cancer cells during EMT in an electrodeformation-based way. For three typical cancer cells, i.e., MCF-7, MDA-MB-231 and A549 cells, our experimental outcome manifests that there exists significant difference in the deformability before and after EMT induced by TGF-beta 1. Both MCF-7 and MDA-MB-231 cells after EMT show more than 40% reduction in elasticity and viscosity, whereas A549 cells appear to have a similar to 25% decrease in elasticity and similar to 35% in viscosity, respectively. These findings imply it is feasible to monitor the EMT process by means of mechanical clue of cells involved. It is expected that the present electrodeformation-based technique can be developed into a label-free, high-throughput method to evaluate metastatic potential of cancer cells, which is crucial to early detection and diagnosis of cancer. (C) 2016 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available