Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 246, Issue -, Pages 623-630Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.02.134
Keywords
Electrochemical immunosensor; Microcystin-LR; Silver deposition; Polypyrrole microsphere; Gold nanoparticle; Signal amplification
Funding
- National Science Foundation of China [21105006]
- Natural Science Foundation of Changzhou [CE20165047]
- State Key Laboratory of Analytical Chemistry for Life science [SKLACLS1403]
- Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology [BM2012110]
- Jiangsu Key Laboratory of Materials Surface Science and Technology
- Jiangsu Collaborative Innovation Center of Photovoltaic Science and Engineering
- Advanced Catalysis and Green Manufacturing Collaborative Innovation Center (Changzhou University) [213164]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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This work designed an ultrasensitive electrochemical immunosensor for microcystin-LR (MC-LR) detection using gold nanoparticle functional polypyrrole microsphere (AuNP/PPyMS) as a novel signal tag to induce silver enhancement for signal amplification. The dispersed polypyrrole microsphere (PPyMS) synthesized with a chemical oxidative polymerization was used to load AuNP for labeling signal antibodies, which enhanced the detection signal due to large surface area. The proposed MC-LR immunosensor was constructed by coating a polyethylene glycol film on a carbon nanotubes modified electrode and then immobilizing MC-LR antigen on the film. With a competitive immunoassay format, coated MC-LR competed for MC-LR antibody with added target MC-LR to form antibody -antigen immunocomplex. Subsequently, the immunocomplex reacted with AuNP/PPyMS labeled signal antibodies. The MC-LR immunoreaction monitoring was achieved by the electrochemical stripping signal of the deposited silver catalyzed by AuNP/PPyMS. AuNP/PPyMS-mediated silver enhancement along with carbon nanotubes-promoted electron transfer led to high detection sensitivity of MC-LR. Under optimal conditions, the MC-LR immunoassay had a wide linear range of 0.25 ngL(-1) to 50 mu gL(-1), low detection limit of 0.1 ng L-1, and good precision, reproducibility and stability. The designed amplification strategy has potential application in the ultrasensitive electrochemical detection of other algae toxins. (c) 2017 Elsevier B.V. All rights reserved.
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