An ultrasensitive sandwich-type electrochemical immunosensor for the determination of SKBR-3 breast cancer cell using rGO-TPA/FeHCFnano labeled Anti-HCT as a signal tag

Here in, a high sensitive sandwich-type electrochemical immunosensor for the determination of SKBR-3 breast cancer cell has been fabricated using of green synthesized reduced graphene oxide as a platform to the immobilization of primary Herceptin antibody (Anti-HCT). The various reduced graphene oxide-tetrasodium 1, 3, 6, 8-pyrenetetrasulfonic acid/metal hexacyanoferrates (rGO-TPA/MHCFnano) nanocomposites included rGO-TPA/FeHCF, rGO-TPA/CoHCF, rGO-TPA/NiHCF and rGO-TPA/CuHCF were used as electrochemical labels of secondary Herceptine antibody. The obtained result showed that the sensitivity of proposed sandwich-type electrochemical immunosensor for the determination of SKBR-3 breast cancer cells (30,000 cells mL(-1)) using rGO-TPA/FeHCFnano labeled secondary Herceptine antibody was higher than the other rGO-TPA/MHCFnano-labeled secondary Herceptine antibodies. The differential pulse voltammetry (DPV) technique was used for the determination of SKBR-3 breast cancer cell in the concentration range of 500-30,000 cells mL-1 with the limit of detection of 21 cells mL-1. The proposed sandwich-type electrochemical immunosensor exhibited high selectivity, liner range responsibility and good stability. (C) 2016 Elsevier B.V. All rights reserved.