Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 253, Issue -, Pages 495-501Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.06.026
Keywords
Photoelectrochemical; GO-ZnO-CdS; T-Hg2+-T; Excitation energy transfer effect; Dye sensitization effect
Funding
- National Natural Science Foundation of China [21605111]
- Natural Science Foundation of Shanxi Province [201601D021037]
- Youth Foundation of Taiyuan University of Technology [2015QN033]
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Mercuric ion (Hg2+) as one kind of environmental pollutants has attracted more and more attentions due to its persistence, mobility and biological enrichment. In this study, a novel photoelectrochemical biosensor for Hg2+ detection was constructed based on dye sensitization effect induced by Y-shaped DNA transformation as signal amplification strategy. Firstly, graphene oxide-ZnO-CdS nanohybrid (GO-ZnO-CdS) as photoelectric material was synthesized by simple hydrothermal method, and was employed to modify ITO electrode. Secondly, Y-shape DNA was labeled by Au NPs in the solution, and which crosslinked on the surface of GO-ZnO-CdS modified ITO electrode by carbodiimide coupling method, which resulted in the excitation energy transfer (EET) effect between Au NPs and CdS. Finally, in the presence of Hg2+, the structure of DNA on the modified electrode changed from Y-shape into hairpin accompanying the proximity of dye molecule based on T-Hg2+-T, which eliminated EET effect and triggered dye sensitization effect for enhancing photocurrent response. Under optimal conditions, the photocurrent linearly increased with the increasing of Hg2+ levels in the range of 5 pM - 500 pM with the detection limit of 1:5 pM(LOD = -3 sigma(blank)/s) More importantly, the developed biosensor showed the advantages of acceptable selectivity, repeatability and stability. (C) 2017 Elsevier B.V. All rights reserved.
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