Journal
SENSORS
Volume 17, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/s17081912
Keywords
cellobiose dehydrogenase; gold nanoparticles; electrodeposition; glucose biosensor; human saliva
Funding
- Swedish Research Council [2014-5908]
- European Commission [FP7-PEOPLE-2013-ITN-607793]
- Erasmus+ Project Unipharma-Graduates
Ask authors/readers for more resources
Efficient direct electron transfer (DET) between a cellobiose dehydrogenase mutant from Corynascus thermophilus (CtCDH C291Y) and a novel glassy carbon (GC)-modified electrode, obtained by direct electrodeposition of gold nanoparticles (AuNPs) was realized. The electrode was further modified with a mixed self-assembled monolayer of 4-aminothiophenol (4-APh) and 4-mercaptobenzoic acid (4-MBA), by using glutaraldehyde (GA) as cross-linking agent. The CtCDH C291Y/GA/4-APh, 4-MBA/AuNPs/GC platform showed an apparent heterogeneous electron transfer rate constant (ks) of 19.4 +/- 0.6 s(-1), with an enhanced theoretical and real enzyme surface coverage (Gamma(theor) and Gamma(real)) of 5287 +/- 152 pmol cm(-2) and 27 +/- 2 pmol cm(-2), respectively. The modified electrode was successively used as glucose biosensor exhibiting a detection limit of 6.2 mu M, an extended linear range from 0.02 to 30 mM, a sensitivity of 3.1 +/- 0.1 mu A mM(-1) cm(-2) (R-2 = 0.995), excellent stability and good selectivity. These performances compared favourably with other glucose biosensors reported in the literature. Finally, the biosensor was tested to quantify the glucose content in human saliva samples with successful results in terms of both recovery and correlation with glucose blood levels, allowing further considerations on the development of non-invasive glucose monitoring devices.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available