Journal
SENSORS
Volume 17, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/s17051132
Keywords
tyrosinase; graphene-gold nanocomposite; electrochemical biosensor; phenolic compounds
Funding
- Malaysian Government through its Ministry of Education under the Fundamental Research Grant Scheme [FRGS/1/2015/SG01/UPM/02/15]
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In this research work, electrochemical biosensor was fabricated based on immobilization of tyrosinase onto graphene-decorated gold nanoparticle/chitosan (Gr-Au-Chit/Tyr) nanocomposite-modified screen-printed carbon electrode (SPCE) for the detection of phenolic compounds. The nanocomposite film was constructed via solution casting method. The electrocatalytic activity of the proposed biosensor for phenol detection was studied using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Experimental parameters such as pH buffer, enzyme concentration, ratio of Gr-Au-Chit, accumulation time and potential were optimized. The biosensor shows linearity towards phenol in the concentration range from 0.05 to 15 mu M with sensitivity of 0.624 mu A/mu M and the limit of detection (LOD) of 0.016 mu M (S/N = 3). The proposed sensor also depicts good reproducibility, selectivity and stability for at least one month. The biosensor was compared with high-performance liquid chromatography (HPLC) method for the detection of phenol spiked in real water samples and the result is in good agreement and comparable.
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