Journal
BIOFOULING
Volume 33, Issue 10, Pages 835-846Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2017.1374378
Keywords
Antimicrobial peptide; Streptococcus mutans; biofilm; dental caries
Funding
- Fundacao Cearense de Apoio ao Desenvolvimento Cientifico e Tecnologico (FUNCAP)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Central Analitica-UFC/CT-INFRA/MCTI-SISNANO/Pro-Equipamentos CAPES
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The aims of this study were to describe the synthesis of a novel synthetic peptide based on the primary structure of the KR-12 peptide and to evaluate its antimicrobial and anti-biofilm activities against Streptococcus mutans. The antimicrobial effect of KR-12 and [W-7]KR12-KAEK was assessed by determining the minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations. The evaluation of anti-biofilm activity was assessed through total biomass quantification, colony forming unit counting and scanning electron microscopy. [W-7]KR12-KAEK showed MIC and MBC values ranging from 31.25 to 7.8 and 62.5 to 15.6 mu g ml(-1), respectively. Furthermore, [W-7]KR12-KAEK significantly reduced biofilm biomass (50-100%). Regarding cell viability, [W-7]KR12-KAEK showed reductions in the number of CFUs at concentrations ranging from 62.5 to 7.8 mu g ml(-1) and 500 to 62.5 mu g ml(-1) with respect to biofilm formation and preformed biofilms, respectively. SEM micrographs of S. mutans treated with [W-7]KR12-KAEK suggested damage to the bacterial surface. [W-7]KR12-KAEK is demonstrated to be an antimicrobial agent to control microbial biofilms.
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