Journal
JOURNAL OF ADVANCED PHARMACEUTICAL TECHNOLOGY & RESEARCH
Volume 8, Issue 1, Pages 19-24Publisher
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/2231-4040.197371
Keywords
Anticancer; antidiabetic; Mangifera indica L.; mangiferin
Categories
Funding
- Chulalongkorn University fund (Ratchadaphiseksomphot Endowment Fund) [GCUGR1125592015D]
- Mae Fah Luang University scholarship [MFU002/2555]
Ask authors/readers for more resources
Diabetes and cancer are a major global public health problem. Plant-derived agents with undesirable side-effects were required. This study aimed to evaluate antidiabetic and anticancer activities of the ethanolic leaf extract of Mangifera indica cv. Okrong and its active phytochemical compound, mangiferin. Antidiabetic activities against yeast alpha-glucosidase and rat intestinal alpha-glucosidase were determined using 1 mM of p-nitro phenyl-alpha-D-glucopyranoside as substrate. Inhibitory activity against porcine pancreatic alpha-amylase was performed using 1 mM of 2-chloro-4 nitrophenol-alpha-D-maltotroside-3 as substrate. Nitrophenol product was spectrophotometrically measured at 405 nm. Anticancer activity was evaluated against five human cancer cell lines compared to two human normal cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Mango leaf extract and mangiferin exhibited dose-dependent inhibition against yeast alpha-glucosidase with the IC50 of 0.0503 and 0.5813 mg/ml, respectively, against rat alpha-glucosidase with the IC50 of 1.4528 and 0.4333 mg/ml, respectively, compared to acarbose with the IC50 of 11.9285 and 0.4493 mg/ml, respectively. For anticancer activity, mango leaf extract, at >= 200 mu g/ml showed cytotoxic potential against all tested cancer cell lines. In conclusion, mango leaf possessed antidiabetic and anticancer potential in vitro.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available