Kinetics of transport through the nuclear pore complex

Single molecule microscopy techniques allow to visualize the translocation of single transport receptors and cargo molecules or particles through nuclear pore complexes. These data indicate that cargo molecule import into the nucleus takes less than 10 ms and nuclear export of messenger RNA (mRNA) particles takes 50-350 ms, up to several seconds for extremely bulky particles. This review summarizes and discusses experimental results on transport of nuclear transport factor 2 (NTF2), importin beta and mRNA particles. Putative regulatory functions of importin beta for the NPC transport mechanism and the RNA helicase Dbp5 for mRNA export kinetics are discussed. (C) 2017 Elsevier Ltd. All rights reserved.