Compositional Monosaccharide Analysis of Morus nigra Linn by HPLC and HPCE Quantitative Determination and Comparison of Polysaccharide from Morus nigra Linn by HPCE and HPLC

Background: Morus nigra Linn is not only treated as health food but also medicine in Chinese history. Objective: Here, we have extracted and separated the heteroglycan to monosaccharides of the dry fruits. Method: After heteroglycan being hydrolysised, then were we derivatived the monosaccharides with 1-phenyl- 3-methyl-5-pyrazolone (PMP), and then subsequently HPCE and HPLC were used to separate. HPCE, which held an uncoated capillary (d= 75 mu m) and detected by PDA at 245 nm with borate buffer, the voltage was set at 15 kV and capillary temperature was 25 degrees C. There is Yilite column (Hypersil BDS C18 5 mu m, 4.6mm x 250mm) with HPLC and detected by UV at 245 nm and capillary temperature 25 degrees C. The High Performance capillary electrophoresis method and High Performance Liquid Chromatography method were compared on monosaccharide composition and quantitative determination of polysaccharide from the black mulberry. Results: Through several indicators including standard curve, precision, reproducibility, stability and recovery rate, the result of the two methods is basically consistent while they have complementary advantages. Conclusion: Both methods are suitable for the determination of the black mulberry polysaccharide, but each has its own merits.