Journal
ENEURO
Volume 4, Issue 1, Pages -Publisher
SOC NEUROSCIENCE
DOI: 10.1523/ENEURO.0245-16.2017
Keywords
behavioral analysis; closed-loop behavior; head-fixed behavior; real-time behavior; sensorimotor integration; whisker kinematics
Categories
Funding
- European Research Council (ERC) [670118]
- Human Brain Project [720270]
- Marie Curie fellowship
- Einstein stiftung
- DFG
- Neurocure Center for Excellence
- European Research Council (ERC) [670118] Funding Source: European Research Council (ERC)
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Here, we describe an automated optical method for tracking animal behavior in both head-fixed and freely moving animals, in real time and offline. It takes advantage of an off-the-shelf camera system, the Pixy camera, designed as a fast vision sensor for robotics that uses a color-based filtering algorithm at 50 Hz to track objects. Using customized software, we demonstrate the versatility of our approach by first tracking the rostro-caudal motion of individual adjacent row (D1, D2) or arc whiskers (beta, gamma), or a single whisker and points on the whisker pad, in head-fixed mice performing a tactile task. Next, we acquired high-speed video and Pixy data simultaneously and applied the pixy-based real-time tracking to high-speed video data. With this approach, we expand the temporal resolution of the Pixy camera and track motion (post hoc) at the limit of high-speed video frame rates. Finally, we show that this system is flexible: it can be used to track individual whisker or limb position without any sophisticated object tracking algorithm, it can be used in many lighting conditions including infrared (IR); it can be used to track head rotation and location of multiple animals simultaneously. Our system makes behavioral monitoring possible in virtually any biological setting.
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