Journal
2ND INTERNATIONAL CONFERENCE ON TROPICAL AND COASTAL REGION ECO DEVELOPMENT 2016
Volume 55, Issue -, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1755-1315/55/1/012057
Keywords
Conventional extraction; alternative extraction; brown algae; bioactivities; polyphenols
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Funding
- Directorate of Higher Education (DIKTI)
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Sargassum has been well acknowledged for the potential natural product of its phlorotannins. Solid-liquid extraction (SLE) is the most common method used to extract them. However, this method has some drawbacks such as low yield and toxic. An alternative ecofriendly method has been proposed, i.e. enzyme-assisted extraction (EAE), proven to be more efficient. The aim is to compare the efficiency of SLE and EAE concerning their extraction yield, total phenolic content and antioxidant activity. S. aquifolium, S. ilicifolium and S. polycystum were extracted using water, methanol, methanol 50%, and ethanol 75% and enzymes (Viscozyme and Protamex). Total phenolic content (TPC) was analyzed by Folin-Ciocalteu and antioxidant activity via DPPH and FRAP analysis. This study implied that bioactivity of Sargassum extracted with enzymes is better compared to the one using organic solvents.
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