Journal
SCIENCE TRANSLATIONAL MEDICINE
Volume 9, Issue 388, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/scitranslmed.aag0538
Keywords
-
Categories
Funding
- National Institute of Allergy and Infectious Diseases (NIAID) of the NIH [RO1AI067380, R01AI0833680, R21AI129464, R01AI099631, R21AI129488-01]
- NIH/NIAID International Collaborations in Infectious Disease Research Program [U01AI088647]
- Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco [APQ-0302-4.01/13]
- European Union [(FP7-281803 IDAMS) (41)]
- Fogarty Training Grant [2D43TW001130-08]
- Centers for Disease Control and Prevention
- Department of Microbiology, Immunology, and Pathology
- College of Veterinary Medicine and Biomedical Sciences at Colorado State University
- Office of the Vice President for Research, Colorado State University
Ask authors/readers for more resources
Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available