Journal
DYES AND PIGMENTS
Volume 136, Issue -, Pages 830-835Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2016.09.047
Keywords
Direct visualisation; Fluorescent; Bisulfite; Coumarin
Funding
- Hebei Province Science Foundation for postdoctorals [B2015005001]
- Hebei Province Higher school science research fund for Youths [QN2016112]
- National Science Foundation for Post-doctoral Scientists of China [2016M591402]
- Hebei Province Science Foundation for Youths [B2014201164]
- Hebei university Science Foundation for High-level personnel
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A novel direct visualisation, fluorescent probe derived from coumarin for bisulfate ion detection has been designed and synthesized. This probe shows a rapid, high selectivity, and sensitivity in the detection of bisulfate ion. In a solution of DMSO:HEPES (1:9, v:v, 10 mM, pH 7.4) buffer containing the probe, the presence of HSO3- made the UV-vis absorption of the probe blue-shift from pink to yellow, and the fluorescence of the probe also changed from red to green. Other investigated analytes and ions did not disturb the determination of HSO3-. The reaction mechanism of the determination was proposed based on the nucleophilic attack toward electron-poor C = C bond of the probe. This probe has been successfully applied for determination of HSO3- in living cells. (C) 2016 Elsevier Ltd. All rights reserved.
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