Journal
ENDOCRINE JOURNAL
Volume 64, Issue 7, Pages 663-673Publisher
JAPAN ENDOCRINE SOC
DOI: 10.1507/endocrj.EJ16-0542
Keywords
Calcitriol; Non-alcoholic fatty liver disease; Nuclear factor-erythroid-2-related factor 2; Oxidative stress
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Funding
- Natural Science Foundation of China [30973040]
- TMUGH [ZYYFY2014040]
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The purpose of this study was to determine whether treatment using the active form of vitamin D (1,25(OH)(2)D-3) could protect against high-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) in rats and ameliorate oxidative stress. Male Sprague-Dawley rats were divided into three groups and treated with standard chow, HFD, or HFD plus intraperitoneal injection of 1,25(OH)(2)D-3 (5 mu g/kg body weight, twice per week), respectively, for 16 weeks. Serum lipid profiles, hepatic function, intrahepatic lipid, and calcium levels were determined. Hepatic histology was examined using hematoxylin/eosin, Masson's trichrome, and Oil Red O staining. Oxidative stress was assessed by measuring hepatic malondialdehyde (MDA) and F2 alpha-isoprostane content. Expression of nuclear factor-erythroid-2-related factor 2 (Nrf2) and downstream target genes was analyzed using quantitative RT-PCR. 1,25(OH)(2)D-3 treatment improved the serum lipid profile, reduced intrahepatic lipid levels, and attenuated hepatic steatosis and inflammation in HFD rats. Furthermore, MDA and F2 alpha-isoprostane levels in liver tissue were reduced by 1,25(OH)(2)D-3 administration. Although 1,25(OH)(2)D-3 did not regulate the expression of Nrf2 mRNA, it did induce Nrf2 nuclear translocation. The expression of Nrf2 target genes, including Gclc, Nqo1, Sod2, and Cat, was up-regulated by 1,25(OH)(2)D-3. We conclude that 1,25(OH)(2)D-3 protects against HFD-induced NAFLD by attenuating oxidative stress, inducing NRF2 nuclear translocation, and up-regulating the expression of genes encoding antioxidant enzymes.
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