Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 583, Issue -, Pages 222-227Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scitotenv.2017.01.056
Keywords
Imidaclothiz; Monoclonal antibody; Fluorescence immunoassay; Quantum dots-based fluoroimmunoassay; Time-resolved fluoroimmunoassay
Categories
Funding
- National Natural Science Foundation of China [31431794]
- Doctoral Program of Higher Education Research Fund [20130097120006]
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A direct quantum dots-based fluoroimmunoassay (QDFIA) and a time-resolved fluoroimmunoassay (TRFIA) for imidaclothiz (IMI) were developed by using the quantum dots (QDs)-labeled antibody and the europium (Eu3+)-labeled antibody, respectively. After optimization, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC10) are 20.41 and 0.52 mu g L-1 for the QDFIA, While 6.91 and 0.018 mu g L-1 for the TRFIA, respectively. The cross-reactivities (CRs) with the analogues are negligible except for imidacloprid with CRs of 29.0% for the QDFIA and 26.6% for the TRFIA. The spiked recoveries of IMI in paddy water, soil, pear, tomato, rice, apple, cabbage and cucumber are 72.7-117.6% with a standard deviation (RSD) of 2.4-13.5% for the QDFIA, and 81.3-117.7% with a RSD of 1.6-7.5% for TRFIA. The detection results of the analyses for the real paddy water and pear samples are markedly correlated with these of high-performance liquid chromatography (HPLC). (c) 2017 Elsevier B.V. All rights reserved.
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