Journal
SCIENCE CHINA-LIFE SCIENCES
Volume 60, Issue 5, Pages 506-515Publisher
SCIENCE PRESS
DOI: 10.1007/s11427-017-9008-8
Keywords
CRISPR/Cas9; genome editing; agronomic genes; rice
Categories
Funding
- National Natural Science Foundation of China [31271681, 3140101312]
- Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences
- Jiangsu Agriculture Science and Technology Innovation Fund [CX(13)5075]
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The clustered regularly interspaced short palindromic repeats (CRISPR)-associated endonuclease 9 (CRISPR/Cas9) system has emerged as a promising technology for specific genome editing in many species. Here we constructed one vector targeting eight agronomic genes in rice using the CRISPR/Cas9 multiplex genome editing system. By subsequent genetic transformation and DNA sequencing, we found that the eight target genes have high mutation efficiencies in the T-0 generation. Both heterozygous and homozygous mutations of all editing genes were obtained in T-0 plants. In addition, homozygous sextuple, septuple, and octuple mutants were identified. As the abundant genotypes in T-0 transgenic plants, various phenotypes related to the editing genes were observed. The findings demonstrate the potential of the CRISPR/Cas9 system for rapid introduction of genetic diversity during crop breeding.
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