Journal
SCIENCE
Volume 355, Issue 6329, Pages 1040-1044Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaf4557
Keywords
-
Categories
Funding
- NSF [MCB-0718846, MCB-1026068, MCB-1616111, MCB-0546446, MCB-1445545]
- Department of Energy [DE-FG02097ER25308]
- NSERC
- Microsoft Research
- Biotechnology and Biological Sciences Research Council [BB/M005690/1] Funding Source: researchfish
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1445545, 1616111] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1445537] Funding Source: National Science Foundation
- BBSRC [BB/M005690/1] Funding Source: UKRI
Ask authors/readers for more resources
We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome designwas implemented with BioStudio, an open-source framework developed for eukaryotic genome design, which coordinates design modifications from nucleotide to genome scales and enforces version control to systematically track edits. To achieve complete Sc2.0 genome synthesis, individual synthetic chromosomes built by Sc2.0 Consortium teams around the world will be consolidated into a single strain by endoreduplication intercross. Chemically synthesized genomes like Sc2.0 are fully customizable and allow experimentalists to ask otherwise intractable questions about chromosome structure, function, and evolution with a bottom-up design strategy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available