Journal
SCIENCE
Volume 356, Issue 6340, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aal3321
Keywords
-
Categories
Funding
- Knut and Alice Wallenberg Foundation
- Erling Persson Foundation
- Science for Life Laboratory
- Wellcome Trust [108441/Z/15/Z]
- Alexander S. Onassis Public Benefit Foundation
- Foundation for Education and European Culture
- Embiricos Trust Scholarship of Jesus College Cambridge
- Biotechnology and Biological Sciences Research Council [BB/K00137X/1, BB/G024618/1, BB/H024247/1] Funding Source: researchfish
- Novo Nordisk Fonden [NNF10CC1016517] Funding Source: researchfish
- BBSRC [BB/H024247/1, BB/K00137X/1, BB/G024618/1] Funding Source: UKRI
- Wellcome Trust [108441/Z/15/Z] Funding Source: Wellcome Trust
Ask authors/readers for more resources
Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease. Here we present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localization of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of the proteomes of 13 major organelles. Exploration of the proteomes revealed single-cell variations in abundance or spatial distribution and localization of about half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human cell.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available