Journal
SCIENCE
Volume 358, Issue 6369, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aao2648
Keywords
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Categories
Funding
- Office of Naval Research [N000141010827, N000141310593, N000141410610, N000141612182, N000141612410, N000141512689]
- Army Research Office [W911NF1210238]
- National Science Foundation [CCF1054898, CMMI1333215, CMMI1334109, CMMI1344915, CCF1317291]
- National Institutes of Health [1R01EB01865901]
- NSF [1360635, 1563799, 1334109]
- Autodesk Life Sciences
- China Scholarship Council [201506340048]
- Fannie and John Hertz Foundation
- U.S. Department of Defense (DOD) [N000141612182, N000141512689, N000141612410, W911NF1210238] Funding Source: U.S. Department of Defense (DOD)
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1334109] Funding Source: National Science Foundation
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [1564025, 1563799] Funding Source: National Science Foundation
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [1360635] Funding Source: National Science Foundation
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Self-folding of an information-carrying polymer into a defined structure is foundational to biology and offers attractive potential as a synthetic strategy. Although multicomponent self-assembly has produced complex synthetic nanostructures, unimolecular folding has seen limited progress. We describe a framework to design and synthesize a single DNA or RNA strand to self-fold into a complex yet unknotted structure that approximates an arbitrary user-prescribed shape. We experimentally construct diverse multikilobase single-stranded structures, including a similar to 10,000-nucleotide (nt) DNA structure and a similar to 6000-nt RNA structure. We demonstrate facile replication of the strand in vitro and in living cells. The work here thus establishes unimolecular folding as a general strategy for constructing complex and replicable nucleic acid nanostructures, and expands the design space and material scalability for bottom-up nanotechnology.
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