Journal
SCIENCE
Volume 356, Issue 6334, Pages 205-208Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aak9867
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Funding
- JSPS KAKENHI [JP25116002, JP25116010, JP25116003, JP15K18515, JP15H02042, JP16H01306]
- CREST, JST
- Japan Agency for Medical Research and Development (AMED)
- Waseda Research Institute for Science and Engineering
- JSPS [16J06850]
- Project for Construction of the Basis for the Advanced Materials Science and Analytical Study by the Innovative Use of Quantum Beam and Nuclear Sciences in the Research Reactor Institute of Kyoto University
- Grants-in-Aid for Scientific Research [25116010, 17K19356, 16K18528, 16K18479, 16H01550, 25116002, 16H01306, 25116003, 17K15043, 17H03608, 16J06850, 15H02042, 17K07313, 16H01577] Funding Source: KAKEN
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Nucleosomes are dynamic entities that are repositioned along DNA by chromatin remodeling processes. A nucleosome repositioned by the switch-sucrose nonfermentable (SWI/SNF) remodeler collides with a neighbor and forms the intermediate overlapping dinucleosome. Here, we report the crystal structure of the overlapping dinucleosome, in which two nucleosomes are associated, at 3.14-angstrom resolution. In the overlapping dinucleosome structure, the unusual hexasome nucleosome, composed of the histone hexamer lacking one H2A-H2B dimer from the conventional histone octamer, contacts the canonical octasome nucleosome, and they intimately associate. Consequently, about 250 base pairs of DNA are left-handedly wrapped in three turns, without a linker DNA segment between the hexasome and octasome moieties. The overlapping dinucleosome structure may provide important information to understand how nucleosome repositioning occurs during the chromatin remodeling process.
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