Journal
SCIENCE
Volume 355, Issue 6329, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaf3981
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Funding
- National Natural Science Foundation of China [31471254, 21621004, 21390203]
- Chinese Ministry of Science and Technology [2012CB725201, 2012AA02A708]
- Ph.D. Programs Foundation of Ministry of Education of China [20110002120055]
- Research Fund for the Doctoral Program of Higher Education of China [20120002110022]
- Tsinghua University Initiative grant [2011Z02296]
- NSF [MCB-1026068, MCB-1158201, MCB-1445545]
- BBSRC [BB/M005690/1] Funding Source: UKRI
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1445545] Funding Source: National Science Foundation
- Biotechnology and Biological Sciences Research Council [BB/M005690/1] Funding Source: researchfish
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We designed and synthesized a 976,067-base pair linear chromosome, synXII, based on native chromosome XII in Saccharomyces cerevisiae. SynXII was assembled using a twostep method, specified by successive megachunk integration and meiotic recombinationmediated assembly, producing a functional chromosome in S. cerevisiae. Minor growth defect bugs detected in synXII, caused by deletion of tRNA genes, were rescued by introducing an ectopic copy of a single tRNA gene. The ribosomal gene cluster (rDNA) on synXII was left intact during the assembly process and subsequently replaced by a modified rDNA unit used to regenerate rDNA at three distinct chromosomal locations. The signature sequences within rDNA, which can be used to determine species identity, were swapped to generate a Saccharomyces synXII strain that would be identified as Saccharomyces bayanus by standard DNA barcoding procedures.
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