Journal
SCIENCE
Volume 355, Issue 6329, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaf4597
Keywords
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Funding
- European Research Council (ERC) under the 7th Framework Program (FP7, ERC) [260822]
- Agence Nationale pour la Recherche (ANR) [MeioRec ANR-13-BSV6-0012-02]
- ERASynBio
- ANR [IESY ANR-14-SYNB-0001-03]
- Pasteur-Roux Fellowships
- National Science Foundation of China [31471254]
- Tsinghua University Initiative Scientific Research Program [2011Z02296]
- Ph.D. Programs Foundation of Ministry of Education of China [20110002120055]
- Chinese Ministry of Science and Technology [2012CB725201]
- Natural Science Foundation of China [21621004, 21390203]
- University of Edinburgh
- Scottish Universities Life Sciences Alliance
- U.K. Biotechnology and Biological Sciences Research Council [BB/M005690/1, BB/M025640/1, BB/M00029X/1]
- Shenzhen Engineering Laboratory for Clinical Molecular Diagnostic Promotion [JZF] [(2016)884]
- U.S. National Science Foundation [MCB-0718846, MCB-1026068, MCB-0546446, MCB-1445545]
- BBSRC [BB/M005690/2, BB/M00029X/1, BB/M025640/1, BB/M005690/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/M00029X/1, BB/M005690/1, BB/M005690/2, BB/M025640/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [1419736] Funding Source: researchfish
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1445545, 1445537] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1616111] Funding Source: National Science Foundation
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Although the design of the synthetic yeast genome Sc2.0 is highly conservative with respect to gene content, the deletion of several classes of repeated sequences and the introduction of thousands of designer changes may affect genome organization and potentially alter cellular functions. We report here the Hi-C-determined three-dimensional (3D) conformations of Sc2.0 chromosomes. The absence of repeats leads to a smoother contact pattern and more precisely tractable chromosome conformations, and the largescale genomic organization is globally unaffected by the presence of synthetic chromosome(s). Two exceptions are synIII, which lacks the silent mating-type cassettes, and synXII, specifically when the ribosomal DNA is moved to another chromosome. We also exploit the contact maps to detect rearrangements induced in SCRaMbLE (synthetic chromosome rearrangement and modification by loxP-mediated evolution) strains.
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