Journal
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
Volume 28, Issue 2, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1361-6439/aa9c9c
Keywords
hollow microneedle; biosensor; therapeutic drug monitoring; optofluidic sensing; enzyme-linked assay
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Funding
- Vanier Canada Graduate Scholarships program
- Michael Smith Foreign Study Supplement awards program of the Natural Sciences and Engineering Research Council of Canada
- Swiss National Science Foundation (SNF) Ambizione project [PZ00P2_142511]
- Canada Research Chairs program
- Swiss National Science Foundation (SNF) [PZ00P2_142511] Funding Source: Swiss National Science Foundation (SNF)
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A hollow metallic microneedle is integrated with microfluidics and photonic components to form a microneedle-optofluidic biosensor suitable for therapeutic drug monitoring (TDM) in biological fluids, like interstitial fluid, that can be collected in a painless and minimally-invasive manner. The microneedle inner lumen surface is bio-functionalized to trap and bind target analytes on-site in a sample volume as small as 0.6 nl, and houses an enzyme-linked assay on its 0.06 mm(2) wall. The optofluidic components are designed to rapidly quantify target analytes present in the sample and collected in the microneedle using a simple and sensitive absorbance scheme. This contribution describes how the biosensor components were optimized to detect in vitro streptavidin-horseradish peroxidase (Sav-HRP) as a model analyte over a large detection range (0-7.21 mu M) and a very low limit of detection (60.2 nM). This biosensor utilizes the lowest analyte volume reported for TDM with microneedle technology, and presents significant avenues to improve current TDM methods for patients, by potentially eliminating blood draws for several drug candidates.
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