Journal
CHEMICAL PAPERS
Volume 72, Issue 1, Pages 71-79Publisher
SPRINGER INTERNATIONAL PUBLISHING AG
DOI: 10.1007/s11696-017-0258-9
Keywords
Microcystin-leucine-arginine; Electrochemical; Immunosensor; Graphene oxide; Differential pulse voltammetry (DPV)
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Funding
- National Natural Science Foundation of China [81273130, 41576098]
- Zhejiang Provincial Natural Science Foundation of China [LY13B070013]
- Science and Technology Plan Project of Ningbo City [2012C50043]
- K.C. Wong Magna Fund in Ningbo University, P.R. China
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In this paper, a label-free electrochemical immunosensor for ultrasensitive detection of microcystin-leucine-arginine (MC-LR) based on multi-functionalized graphene oxide was constructed. The graphene oxide has a large surface area for the immobilization of the antibody. Meanwhile, the introduction of the AuNPs and 1-butyl-3-methylimidazolium hexafluorophosphate could enhance the response of the current by improving the electrical conductivity. Thus the electrochemical immunosensor could be prepared through a one-step process and differential pulse voltammetry was employed to detect sensitively MC-LR. Under optimal conditions, the current response of the immunosensor decreased proportionally to the logarithmic concentrations of MC-LR in the range of 0.1-1000 ng/mL with a detection limit of 0.1 ng/mL (S/N = 3). This one-step label-free electrochemical immunosensor showed good performance in specificity, stability, reproducibility, and application.
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