Journal
SCIENCE
Volume 355, Issue 6330, Pages 1184-1187Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaj2103
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Funding
- National Institutes of Health [GM31105]
- ETH Zurich
- European Research Council project BarrAge
- iPHD fellowship from SystemsX.ch
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In yeast, heterochromatin silencing is reported to decline in aging mother cells, causing sterility in old cells. This process is thought to reflect a decrease in the activity of the NAD(+) (oxidized nicotinamide adenine dinucleotide)-dependent deacetylase Sir2. We tested whether Sir2 becomes nonfunctional gradually or precipitously during aging. Unexpectedly, silencing of the heterochromatic HML and HMR loci was not lost during aging. Old cells could initiate a mating response; however, they were less sensitive to mating pheromone than were young cells because of age-dependent aggregation of Whi3, an RNA-binding protein controlling S-phase entry. Removing the polyglutamine domain of Whi3 restored the pheromone sensitivity of old cells. We propose that aging phenotypes previously attributed to loss of heterochromatin silencing are instead caused by aggregation of the Whi3 cell cycle regulator.
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