Journal
SCIENCE
Volume 357, Issue 6357, Pages 1299-+Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aan2399
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Funding
- Swiss National Science Foundation [158999]
- European Molecular Biology Organization (EMBO) [ALTF 306-2016]
- European Research Council [StG-2014-638142]
- Henry Chanoch Krenter Institute for Biomedical Imaging and Genomics
- Leir Charitable Foundations
- Richard Jakubskind Laboratory of Systems Biology
- Lord Sieff of Brimpton Memorial Fund
- I-CORE program of the Planning and Budgeting Committee
- Israel Science Foundation [1902/12, 1796/12, 1486/16]
- EMBO
- European Research Council under the European Union-ERC [335122]
- European Research Council (ERC) [335122] Funding Source: European Research Council (ERC)
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Asymmetric messenger RNA (mRNA) localization facilitates efficient translation in cells such as neurons and fibroblasts. However, the extent and importance of mRNA polarization in epithelial tissues are unclear. Here, we used single-molecule transcript imaging and subcellular transcriptomics to uncover global apical-basal intracellular polarization of mRNA in the mouse intestinal epithelium. The localization of mRNAs did not generally overlap protein localization. Instead, ribosomes were more abundant on the apical sides, and apical transcripts were consequently more efficiently translated. Refeeding of fasted mice elicited a basal-toapical shift in polarization of mRNAs encoding ribosomal proteins, which was associated with a specific boost in their translation. This led to increased protein production, required for efficient nutrient absorption. These findings reveal a posttranscriptional regulatory mechanism involving dynamic polarization of mRNA and polarized translation.
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