Journal
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
Volume 79, Issue 2, Pages -Publisher
WILEY
DOI: 10.1111/aji.12805
Keywords
early-onset pre-eclampsia; MAIT cells; PD-1; pregnancy; TIM-3
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Funding
- National Research, Development and Innovation Office [NKFIH K119529, PD112465]
- TAMOP [4.2.4.A/2-11-1-2012-0001]
- Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences
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ProblemThe objective of this study was to compare the expressions of different immune-checkpoint molecules by MAIT and MAIT-like cells in healthy pregnancy and in early-onset pre-eclampsia. Method of studyPeripheral blood mononuclear cells (PBMC) were stained with monoclonal antibodies to characterize MAIT and MAIT-like cells. Flow cytometric analyses were used to measure PD-1, TIM-3, activation markers, and intracellular perforin expression. ResultsWe identified CD3+/CD8+/V7.2+/CD161++ MAIT cells and a minor cell population characterized by CD3+/CD8+/V7.2+/CD161(lo) surface markers. In measuring the expression of PD-1 receptor, we found a significantly lower expression by MAIT cells in women with early-onset pre-eclampsia. CD69 expression by MAIT cells was significantly elevated in early-onset pre-eclamptic patients. Intracellular perforin content by MAIT and PD-1+ MAIT cells was significantly increased in pre-eclamptic patients compared with healthy individuals. ConclusionAltered frequency and reduced PD-1 expression combined together with the elevated perforin content of MAIT cells insinuate their potential roles in the pathogenesis of early-onset pre-eclampsia.
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