Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 410, Issue 3, Pages 933-941Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-017-0548-7
Keywords
Microfluidics/microfabrication; Capillary electrophoresis/electrophoresis; Clinical/biomedical analysis; Separations/instrumentation; Extraction (SFE vertical bar SPE vertical bar SPME)
Funding
- National Institutes of Health [R01 EB006124]
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We have developed multichannel integrated microfluidic devices for automated preconcentration, labeling, purification, and separation of preterm birth (PTB) biomarkers. We fabricated multilayer poly(dimethylsiloxane)-cyclic olefin copolymer (PDMS-COC) devices that perform solid-phase extraction (SPE) and microchip electrophoresis (mu CE) for automated PTB biomarker analysis. The PDMS control layer had a peristaltic pump and pneumatic valves for flow control, while the PDMS fluidic layer had five input reservoirs connected to microchannels and a mu CE system. The COC layers had a reversed-phase octyl methacrylate porous polymer monolith for SPE and fluorescent labeling of PTB biomarkers. We determined mu CE conditions for two PTB biomarkers, ferritin (Fer) and corticotropin-releasing factor (CRF). We used these integrated microfluidic devices to preconcentrate and purify off-chip-labeled Fer and CRF in an automated fashion. Finally, we performed a fully automated on-chip analysis of unlabeled PTB biomarkers, involving SPE, labeling, and mu CE separation with 1 h total analysis time. These integrated systems have strong potential to be combined with upstream immunoaffinity extraction, offering a compact sample-to-answer biomarker analysis platform.
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