Journal
ARCHIVES OF VIROLOGY
Volume 163, Issue 2, Pages 545-548Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-017-3618-4
Keywords
LAMP; ornamental plant; Potyvirus; virus detection
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Funding
- Shanghai Agriculture Applied Technology Development Program, China [G2014070203]
- Agriculture Research System of Shanghai, China [201708]
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Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMoV, with no cross-reaction with other lily viruses. The sensitivity of LMoV using the LAMP assay was 100 times more sensitive than that using conventional polymerase chain reaction. A reverse transcription LAMP (RT-LAMP) was then successfully applied to detect LMoV RNA. The newly established LAMP and one-step RT-LAMP provide an alternative method for detecting LMoV in lily plants.
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