Journal
ENVIRONMENTAL SCIENCE & TECHNOLOGY
Volume 49, Issue 19, Pages 11356-11362Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.5b02003
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Funding
- National Natural Science Foundation [21307016]
- Natural Science Foundation of Guangdong, China [2014A030308019, S2013040014438]
- National Science Foundation for Excellent Young Scholars of China [51422803]
- Special Foundation for the Science and Technology Innovation Leaders of Guangdong Province [2014TX01Z038]
- Special Fund for Agro-scientific Research in the Public Interest [201503108]
- Guangdong Provincial Innovative Development of Marine Economy Regional Demonstration Projects [GD2012-D01-002]
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Direct visualization evidence is important for understanding the microbial degradation mechanisms. To track the microbial degradation pathways of azo dyes with different polar characterizations, sensors based on the fluorescence resonance energy transfer (FRET) from 1,8-naphthalimide to azo dyes were synthesized, in which the quenched fluorescence will recover when the azo bond was cleaved. In living cells, the sensor-tracking experiment showed that the low polarity and hydrophobic azo dye can be taken up into the cells and reduced inside the cells, whereas the high polarity and hydrophilic azo dye can be reduced only outside the cells because of the selective permeability of the cell membranes. These results indicated that there were two different bacterial degradation pathways available for different polarity azo dyes. To our knowledge, no fluorescent sensor has yet been designed for illuminating the microbial degradation mechanisms of organic pollutants with different characteristics.
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