Journal
BIOMEDICAL PHYSICS & ENGINEERING EXPRESS
Volume 4, Issue 2, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/2057-1976/aaab93
Keywords
direct laser writing; cell scaffold; 3D fabrication; PEGDA hydrogel; neuro2A cell; multi-photon confocal imaging
Funding
- LAAS-CNRS Carnot
- H2020 European project HOLIFab [760927]
- LAAS-CNRSmicro and nanotechnologies platform member of the FrenchRENATECHnetwork
Ask authors/readers for more resources
3D fabrication techniques are rapidly expanding in the field of scaffold development for cell culture and tissue engineering. Herein we report the realization of free-standing PEGDA hydrogel architectures by using two-photon lithography. The morphological and immunofluorescence characterization of neuro2A cells revealed a tridimensional colonization featuring multiple neuritic extensions per cell as well as the expression of ss-tubulin neuronal marker and actin microfilaments. The results open new perspectives in the continuous quest for structured biomaterials able to provide a favorable environment to cells and at the same time not interfering with imaging protocols necessary for a clear scenario of the cell seeding.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
