Journal
RNA
Volume 23, Issue 6, Pages 882-891Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.060095.116
Keywords
3 ' nontemplated nucleotides addition (3 ' NTA); CD4 T lymphocytes; microRNAs; uridylation; Zcchc11 (TUT4); Zcchc6 (TUT7); isomiRs
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Funding
- Ministerio de Economia y Competitividad-Spain [SAF2014-55579R]
- ERC [294340-GENTRIS]
- COST-Action [BN1202]
- CIBERCV (Instituto de Salud Carlos III) [PIE-13-00041]
- INDISNET [S2011-BMD-2332]
- FEDER
- Pro-CNIC Foundation
- Fondo Europeo de Desarrollo Regional (FEDER)
- Instituto de Salud Carlos III [MS14/00219]
- FPU program (Spanish Ministry of Education)
- Plan Estatal de Investigacion Cientffica y Tecnica y de Innovacion Programa Estatal de I+D+i Orientada a los Retos de la Sociedad Retos Investigacion: Proyectos I+D+i, Ministerio de Economia, Industria y Competitividad [SAF2013-42767-R, SAF2016-75511-R]
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Activation of T lymphocytes requires a tight regulation of microRNA (miRNA) expression. Terminal uridyltransferases (TUTases) catalyze 3' nontemplated nucleotide addition (3'NTA) to miRNAs, which may influence miRNA stability and function. Here, we investigated 3'NTA to mature miRNA in CD4 T lymphocytes by deep sequencing. Upon T-cell activation, miRNA sequences bearing terminal uridines are specifically decreased, concomitantly with down-regulation of TUT4 and TUT7 enzymes. Analyzing TUT4-deficient T lymphocytes, we proved that this terminal uridyltransferase is essential for the maintenance of miRNA uridylation in the steady state of T lymphocytes. Analysis of synthetic uridylated miRNAs shows that 3' addition of uridine promotes degradation of these uridylated miRNAs after T-cell activation. Our data underline post-transcriptional uridylation as a mechanism to fine-tune miRNA levels during T-cell activation.
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