Journal
INVENTIONS
Volume 3, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/inventions3010011
Keywords
3D locomotion; behavior; zebrafish; idTracker; ImageJ
Categories
Funding
- Ministry of Science and Technology [MOST105-2313-B-033-001-MY3]
Ask authors/readers for more resources
Generally, the measurement of three-dimensional (3D) swimming behavior in zebrafish relies on commercial software or requires sophisticated scripts, and depends on more than two cameras to capture the video. Here, we establish a simple and economic apparatus to detect 3D locomotion in zebrafish, which involves a single camera capture system that records zebrafish movement in a specially designed water tank with a mirror tilted at 45 degrees. The recorded videos are analyzed using idTracker, while spatial positions are calibrated by ImageJ software and 3D trajectories are plotted by Origin 9.1 software. This easy setting allowed scientists to track 3D swimming behavior of multiple zebrafish with low cost and precise spatial position, showing great potential for fish behavioral research in the future.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available