Journal
BIOTECHNOLOGY LETTERS
Volume 40, Issue 2, Pages 383-391Publisher
SPRINGER
DOI: 10.1007/s10529-017-2479-y
Keywords
Corynebacterium glutamicum; Gene expression; L-Lysine production; Metabolic engineering; Native promoters; Promoters
Categories
Funding
- National Hi-Tech Research and Development Program of China [2014AA021203]
- Science and Technology Service Network Initiative of the Chinese Academy of Sciences [KFJ-EW-STS-078]
- National Natural Science Foundation of China [31100074]
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To identify useful native promoters of Corynebacterium glutamicum for fine-tuning of gene expression in metabolic engineering. Sixteen native promoters of C. glutamicum were characterized. These promoters covered a strength range of 31-fold with small increments and exhibited relatively stable activity during the whole growth phase using beta-galactosidase as the reporter. The mRNA level and enzymatic activity of the lacZ reporter gene exhibited high correlation (R (2) = 0.96) under the control of these promoters. Sequence analysis found that strong promoters had high similarity of the -10 hexamer to the consensus sequence and preference of the AT-rich UP element upstream the -35 region. To test the utility of the promoter library, the characterized native promoters were applied to modulate the sucCD-encoded succinyl-CoA synthetase expression for l-lysine overproduction. The native promoters with various strengths realize the efficient and precise regulation of gene expression in metabolic engineering of C. glutamicum.
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