Journal
CANCER IMMUNOLOGY IMMUNOTHERAPY
Volume 67, Issue 3, Pages 483-494Publisher
SPRINGER
DOI: 10.1007/s00262-017-2100-1
Keywords
Adoptive transfer NK cells; Cellular therapy; Chemotherapy-refractory non-Hodgkin lymphoma; IL-2; Immunosuppressive environment
Categories
Funding
- NIH [P30 CA77598]
- National Center for Advancing Translational Sciences of the National Institutes of Health Award [UL1TR000114]
- American Society of Hematology Scholar Award (Veronika Bachanova) [P01 CA65493, P01 CA111412, R01 CA72669]
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We report a novel phase 2 clinical trial in patients with poor prognosis refractory non-Hodgkin lymphoma (NHL) testing the efficacy of haploidentical donor natural killer (NK) cell therapy (NK dose 0.5-3.27 x 10(7) NK cells/kg) with rituximab and IL-2 (clinicaltrials.gov NCT01181258). Therapy was tolerated without graft-versus-host disease, cytokine release syndrome, or neurotoxicity. Of 14 evaluable patients, 4 had objective responses (29%; 95% CI 12-55%) at 2 months: 2 had complete response lasting 3 and 9 months. Circulating donor NK cells persisted for at least 7 days after infusion at the level 0.6-16 donor NK cells/mu L or 0.35-90% of total CD56 cells. Responding patients had lower levels of circulating host-derived Tregs (17 +/- 4 vs. 307 +/- 152 cells/mu L; p = 0.008) and myeloid-derived suppressor cells at baseline (6.6 +/- 1.4% vs. 13.0 +/- 2.7%; p = 0.06) than non-responding patients. Lower circulating Tregs correlated with low serum levels of IL-10 (R-2 = 0.64; p < 0.003; n = 11), suggestive of less immunosuppressive milieu. Low expression of PD-1 on recipient T cells before therapy was associated with response. Endogenous IL-15 levels were higher in responders than non-responding patients at the day of NK cell infusion (mean +/- SEM: 30 +/- 4; n = 4 vs. 19.0 +/- 4.0 pg/ml; n = 8; p = 0.02) and correlated with day 14 NK cytotoxicity as measured by expression of CD107a (R-2 = 0.74; p = 0.0009; n = 12). In summary, our observations support development of donor NK cellular therapies for advanced NHL as a strategy to overcome chemoresistance. Therapeutic efficacy may be further improved through disruption of the immunosuppressive environment and infusion of exogenous IL-15.
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