Journal
ACS CHEMICAL BIOLOGY
Volume 13, Issue 2, Pages 475-480Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acschembio.7b00616
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Funding
- German Bundesministerium fur Bildung and Forschung [FKZ 13N14122]
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A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.
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