Journal
REVIEW OF SCIENTIFIC INSTRUMENTS
Volume 88, Issue 1, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.4973917
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Funding
- UK Engineering and Physical Sciences Research Council (EPSRC) [EP/I02770X/1]
- Imperial College London EPSRC Impact Acceleration Account Pathways to Impact grant
- UK Biotechnology and Biological Sciences Research Council (BBSRC) [BB/M006786/1]
- EPSRC
- Institute of Chemical Biology EPSRC funded Doctoral Training Centre
- Biotechnology and Biological Sciences Research Council [BB/M006786/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [1580567, EP/I02770X/1, 1102602] Funding Source: researchfish
- BBSRC [BB/M006786/1] Funding Source: UKRI
- EPSRC [EP/I02770X/1] Funding Source: UKRI
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We report the characterisation of gated optical image intensifiers for fluorescence lifetime imaging, evaluating the performance of several different prototypes that culminate in a new design that provides improved spatial resolution conferred by the addition of a magnetic field to reduce the lateral spread of photoelectrons on their path between the photocathode and microchannel plate, and higher signal to noise ratio conferred by longer time gates. We also present a methodology to compare these systems and their capabilities, including the quantitative readouts of Forster resonant energy transfer. (C) 2017 Author(s).
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