Aidingibacillus halophilus gen. nov., sp nov., a novel member of the family Bacillaceae

A Gram-positive, non-motile, asporogenous and aerobic bacterium, designated YIM 98012(T), was isolated from a salt lake in China. Strain YIM 98012(T) was found to be catalase and oxidase positive. Optimal growth of strain YIM 98012(T) was observed at 37 A degrees C and pH 7.0 and it was found to grow in the presence of 5-20% (w/v) NaCl (optimum 10% NaCl). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel strain is affiliated with the family Bacillaceae of the phylum Firmicutes and that it shares high (94.7%) sequence similarity with Alteribacillus persepolensis DSM 21632(T) and does not show sequence similarities of more than 94.0% to known members of other related genera. The major fatty acids (> 10%) were identified as anteiso-C-15:0, anteiso-C-17:0, iso-C-16:0 and C-16:0. The genomic DNA G+C content was determined to be 41.0 mol% and the dominated respiratory quinone was identified as MK-7. The cell wall peptidoglycan of strain YIM 98012(T) was found to contain meso-diaminopimelic acid, while the polar lipids profile was found to include diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine. Based on physiological and chemotaxonomic characteristics, strain YIM 98012(T) is concluded to be the type strain of the type species of a novel genus in the family Bacillaceae for which the name Aidingibacillus halophilus gen. nov., sp. nov. is proposed. The type strain is YIM 98012(T) (= KCTC 33868(T) = DSM 104332(T)).