Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 41, Issue 4, Pages 501-510Publisher
SPRINGER
DOI: 10.1007/s00449-017-1884-x
Keywords
Alkali lignin; Biodegradation; High-throughput screening; Lipid; Rhodococcus pyridinivorans CCZU-B16
Funding
- Natural Science Foundation of Jiangsu Province [BK20141172]
- Key Laboratory of Fermentation Engineering (Ministry of Education)
- Green Catalysis and Applied Enzyme Group of Changzhou Vocational Institute of Engineering [111308002216006]
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Based on the Prussian blue spectrophotometric method, one high-throughput screening strategy for screening lignin-degrading microorganisms was built on 24-well plate at room temperature. One high activity of alkali lignin-degrading strain Rhodococcus pyridinivorans CCZU-B16 was isolated from soil. After the optimization of biodegradation, 30.2% of alkali lignin (4 g/L) was degraded under the nitrogen-limited condition (30/1 of C/N ratio; g/g) at 30 A degrees C for 72 h. It was found that syringyl (S) units and guaiacyl (G) in lignin decreased after biodegradation. Moreover, the accumulated lipid in cells had a fatty acid profile rich in C16 and C18 with four major constituent fatty acids including palmitic acid (C16:0; 22.4%), palmitoleic acid (C16:1; 21.1%), stearic acid (C18:0; 16.2%), and oleic acid (C18:1; 23.1%). In conclusion, Rhodococcus pyridinivorans CCZU-B16 showed high potential application in future. [GRAPHICS] .
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