Journal
REPRODUCTIVE BIOMEDICINE ONLINE
Volume 34, Issue 3, Pages 298-311Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rbmo.2016.11.011
Keywords
Acrosome reaction; Capacitation; Chromatin maturity; DNA integrity; Hyaladherins; Hyaluronic acid
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Funding
- Forough Torabi through an EU Marie Curie Sklodowsak International Training Network Scholarship
- Cultural Bureau of the Kingdom of Saudi Arabia
- Efficacy and Mechanism Evaluation Programme, an MRC and NIHR partnership [11/14/34]
- Medical Research Council [MC_PC_13092] Funding Source: researchfish
- MRC [MC_PC_13092] Funding Source: UKRI
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Mature spermatozoa bind hyaluronic acid in the extracellular matrix via hyaladherins. Immature spermatozoa may be unable to interact because they do not express the appropriate hyaladherins on their surface. Fresh human semen samples were fractionated using differential density gradient centrifugation (DDGC) and the ability of these fractions to bind hyaluronic acid was evaluated. The presence of sperm hyaladherins was also assessed. CD44 was located mainly on the acrosome and equatorial segment and became more restricted to the equatorial segment in capacitated spermatozoa. Hyaluronic acid-TRITC (hyaluronic acid conjugated with tetramethylrhodamine isothiocyanante), a generic hyaluronic-acid-binding reagent, labelled the membrane and the neck region, particularly after capacitation. Sperm populations obtained after DDGC or after interaction with hyaluronic acid were assessed for DNA fragmentation and chromatin maturity. Strong relationships between both measures and sperm sedimentation and hyaluronic-acid-binding profiles were revealed. Capacitation enhanced hyaluronic acid binding of both DDGC-pelleted sperm and sperm washed free of seminal fluid. In conclusion, hyaladherins were detected on human sperm and a higher capacity for sperm hyaluronic-acid-binding was shown to correspond with their DDGC sedimentation profiles and with lower levels of DNA fragmentation and better chromatin maturity. Capacitation induced changes in the distribution and presence of hyaladherins may enhance hyaluronic-acid-binding. (C) 2016 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
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